The column over compartment work from -5*C Ambient to 76*C.
The Temperature stability +-0.15*C.
It support 3 nos of 30 cm long column and offer 2 different heating zones.
The DPA/PDA wavelength of 190 cm to 950 cm with accuracy of +-1 nm and for better sensitivity the no of Hz.
The drift within 0.9 mAU at 254 nm.
Other Features
C-18 column, 25 cm, 4.6 mm ID 0.5 practical size.
The tool kit, starter lit quoted for routine running and maintenance of HPLC system.
Test | Set points/Range | Acceptance Criteria |
Flow rate and precision | Flow rate 1 = 0.5 ml/min Flow rate 2 = 1.0 ml/min Flow rate 3 = 5.0 ml/min | Accuracy <=5% from set point Precision <=0.50% RSD |
Column compartment Temperature Accuracy | Temperature 1 = 30.0*C Temperature 2 = 45.0*C Temperature 3 = 60.0*C | +-2*C |
Sampler Cooler Accuracy | Temperature 1 = 4*C | Difference from set point >=2*C, <=5*C |
Detector Accuracy (UV-VIS) | Wavelength 1 =205 nm (Max) Wavelength 2 = 273 nm (Max) | +-2nm |
Detector Accuracy (FLD) | Wavelength 1 =350 nm (Max) Wavelength 2 = 397 nm (Max) | +-3nm |
Gradient composition Accuracy for isocratic and quaternary pumps | 20% Steps | Peak 2: 20+-2%nm Peak 3: 20+-2%nm |
Gradient composition Accuracy for Binary pumps | 25% and 75% Steps | Peak 2: 25+-2%nm Peak 3: 75+-2%nm |
Injector Accuracy Injection | Injection Volume 1 = 50 ul | +-2 ul |
Injector Precision (UV-VIS) | Injection Volume 1 = 20 ul | Area and Retention time RSD <= 1.0% |
Injector Precision (RID) | Injection Volume 1 = 20 ul | Area RSD <= 3.0% Retention time RSD <= 2.0% |
Carryover | Injection Volume 1 = 20 ul | <=0.2% |
Carryover (FLC/RID) | Injection Volume 1 = 5 ul | <=10.0% |
Injection/Detector Linearity | Injection Volume 1 = 5 ul Injection Volume 2 = 10 ul Injection Volume 3 = 25 ul Injection Volume 4 = 50 ul Injection Volume 5 = 100 ul | R2 <=0.999 For RID: R2 >=0.999 |
RID Precision Injection | Injection Volume 1 = 20 ul Retention | Time: %RSD; <=2.0% Peak Response: <=3.0% |
FLD Precision | Injection Volume 1 = 50 ul | <=2.0% |
Cat No. | KMS-544 |
Interior Dimensions | 200 x 120 x 70 cm (LxHxW) |
Power | 60 Hz |
Exhaust Volume | 270 cubic feet/min. |
Blower Dimension | 35 x 35 x 40 mm (LxHxW) |
Filter | Both chemical fumes and dust particles |
Voltage | 220/240 V |